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David, We have seen this a number of times with IgM monoclonal components. There was a short paper on this which related to capillary electrophoresis in clinchem some time ago but its message is equally applicable with normal gel electrophoresis. We probably have around We use capillary electrophoresis here at Nottingham city, we always measure Igs turbidimetrically with every request for electrophoresis and vice versa. The way we generally pick this up is by the large discrepancy between the two results. Often this is >25 and the on the electropherogram the peak is much much smaller like the one you reported. On these occasions we treat the sample with mercaptoethanol and re run the electrophoresis. We keep a log of these patients. This seems to work very well. Another clue that may point to the polymerisation of the monoclone is the presence of banding across all lanes on immunofixation. We tend to treat these samples also. Regards Craig Webster Nottingham City Hospital -----Original Message----- From: owner-acb-clin-chem-gen [mailto:owner-acb-clin-chem-gen] Sent: Friday, August 08, 2003 2:23 PM To: cwebste1 Subject: IGM and scanning ---------------------- Information from the mail header ----------------------- Sender: Clinical biochemistry discussion list ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/