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The collection and preservation of urine and plasma samples for the determination of catecholamines was summarized in an article by Boomsma et al in Clin Chem 39:2503-2508, 1993. In summary- Plasma: Centrifugation required within 1 hour of collection of EDTA blood sample (no need for a refrigerated centrifuge). Plasma catecholamines stability: 1 day at 20 C 2 days at 4 C 1 month at -20 C (or 6 months with added glutathione) 1 year at -70 C Urine: - Collection can be carried out without acid (catechols stable for up to 4 days)! - Acidification is the best method for long-term preservation of urinary catecholamines - With EDTA and meta-bisulfite addition, catechols are stable at 4 C for up to one year Preservatives can be added after the collection is complete. We tried out these guidelines in a relatively small series of samples, and our experience confirmed them. Best wishes, Oren Zinder Department of Clinical Biochemistry and Laboratory Medicine Rambam Medical Center Haifa, ISRAEL Prof. Oren Zinder Department of Clinical Biochemistry Rambam Medical Center Haifa, ISRAEL 31096 Tel: +972-4-854-3767 Fax: +972-4-854-2409 email: [log in to unmask] ##################################################################################### This e-mail message has been scanned for Viruses and Content and cleared by MailMarshal ##################################################################################### ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/