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Dear colleagues Does anyone have a well documented (chromatographic) method to separate conjugated steroids from free steroids? I am evaluating pre-analysis extractionmethods for 17 hydroxyprogesteron in young children. To select the best method I need a way to separate the (crossreacting) conjugated steroids from the non-conjugated steroids completely. Literature probably goes back to the sixties. With regards Bart Ballieux Dr. B.E.P.B. Ballieux Clinical Biochemist Leiden University Medical Centre P.O.box 9600 2300 RC Leiden The Netherlands Tel:+3171-5262165/2278 Fax: +3171-5266753 email: [log in to unmask] ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/