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Hi, I've a feeling that the buck stops with me with help on MarsBar. So, buck in hand... Second level analyses with MarsBar: Two approaches: 1) Define the ROIs on single subjects; analyze with MarsBar; Run the Statistic table command on your chosen t contrast, and extract the contrast value. Repeat for each subject; run a single sample t test on the values for the single subjects, maybe using SPSS. 2) Run single subject analyses on normalized data in SPM, and get the relevant contrast images for each subject; define ROI, then run MarsBar random effects model with ROI, and using the contrast images for each subject for scans - this is the standard SPM approach. I hope that's clear, but please do let me know if not. Could you give any more details on the problems with the m files? Thanks a lot, Matthew