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This morning we also got an interference with the Olympus CRP method. The result was 205 mg/l, just 5 mg/L over the reportable range. Therefore the analyser performed a second assay after an automatic dilution that flagged the interference: The second result was 60 mg/l, confirmed by the CRP Roche/Hitachi method. The sample came from an old woman (80 years old) with a strong syndrome of biliary retention (serum bilirubin = 240 mg/l). Usually, these bilirubin concentrations do not interfere in the CRP assay. Something else related to biliary retention could be the explanation. Is there evidence that biliary salts might change the immunoturbidimetric reaction rate. Philippe Marquis Service de biochimie Centre hospitalier régional Metz - France [log in to unmask] www.marquis-soft.com ----- Original Message ----- From: "John Meek" <[log in to unmask]> > We have seen interference (positive and negative) with the Olympus > immunoturbidimetric CRP method from certain neonatal samples. The > interference did not occur with the Olympus high sensitivity latex > method. I have not been able to find the cause of this. It is not > anything obvious such as lipaemia. It is only associated with babies in > the first week or two of life, and fluctuates from day to day. In fact, > it has sometimes seemed to fluctuate on a 2-day cycle. The > paediatricians can not shed any light on it either. ----- Original Message ----- From: "Ball Richard (RC9) Luton & Dunstable Hospital TR" <[log in to unmask]> >We have recently had a problem with high CRP's (out of assay limit) >measured on our Beckman LX20 or CX7 on certain babies from our NICU. >When we sent the samples to another lab using a different method the >results were normal. We then obtained a kit for the Beckman Array >which also gave normal results. >It would appear that there is some interference with the LX20 assay, >which it has been suggested may be due to conjugated bilirubin. >We do not think that this is the whole story as we have also had >babies with high conjugated bilirubin and normal CRP. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/