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The procedure described by Dr. Ballieux is very similar to the procedure we use. Basically ours is: 1. Dip urine with dipstick - if NEG (Report Myo <15 mg/L). If POS then 2. Dilute urine 1:40 with saline and redip - if NEG (Report Myo <15 mg/L). If POS at 1:40 then 3. Pass 1:40 dilution through Amicon Centrifree filter. It has a 30,000 MW cutoff. 4. Dip filtrate. If NEG (Report Myo<15 mg/L). If POS (Report Myo >15 mg/L). This is quick and meets the needs of our clinicians. Anyone using this will need to adjust the dilution factor for the urine to respond to the particular brand of dipstick in use so that the dilution corresponds to 15 mg/L. Also, because this test depends on the peroxidase activity of myoglobin it should be performed on fresh specimens. Hope this is helpful. James D. Peele, Ph.D. Director of Clinical Chemistry Baptist Medical Center 800 Prudential Drive Jacksonville, FL 32207 Voice 904-202-2675 Fax 904-202-2795 E-mail [log in to unmask] >>> Bart Ballieux <[log in to unmask]> 04/11/02 08:31AM >>> We handle a quick and dirty method: - Use a urine screening dipstick and check whether hemoglobin is positive - If positive and no macroscopic hematuria is present, dilute urine 40x with water - check hemoglobin in the urine screening again. - If diluted urine is positive, Myoglobin is > 15 mg/l (using Bayer Multistix ) - If undiluted positive and diluted negative result is reported as Myoglobin < 15 mg/l - If undiluted negative result is reported as negative. Plasma CK should always be requested together with urine myoglobin Dr.Ir. B.E.P.B. Ballieux Klinisch Chemicus Medial, Locatie Spaarne Ziekenhuis Händellaan 2, 2102 CW Heemstede The Netherlands Tel ++3123-5141516 sein 4739 Fax ++3123-5141208 ----- Original Message ----- From: "Mohammad Al-Jubouri" <[log in to unmask]> To: <[log in to unmask]> Sent: Monday, April 08, 2002 4:34 PM Subject: Heavy myoglobinuria and urine colour > Hi every body > > The classical storey is that heavy myoglobinuria is > manifested by dark colour urine. Over this weekend, a > young lady presented with severe rhabdomyolysis ( I > mean really severe with a peak CK of 350,000 IU/L)and > acute renal failure followed. Her urine at peak of CK > showed a myoglobin of > 1600 ug/L but the colour was > not dark, it was only a faint pinkish hue (like a > dilute haemoglobin solution). Does this reflect the > experience of others in similar cases? > > thanks > > Mohammad > > ===== > Dr. M A Al-Jubouri > Consultant Chemical Pathologist > > __________________________________________________ > Do You Yahoo!? > Everything you'll ever need on one web page > from News and Sport to Email and Music Charts > http://uk.my.yahoo.com > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and clinical > community working in clinical biochemistry. > Please note, archived messages are public and can be viewed > via the internet. Views expressed are those of the individual and > they are responsible for all message content. > > ACB Web Site > http://www.acb.org.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ > > ____________________________________________________________________________ _________________ > This inbound message has been checked for all known viruses by KPN IV-Scan, powered by MessageLabs. > For further information visit: http://www.veiliginternet.nl > ____________________________________________________________________________ _________________ _____________________________________________________________________________________________ This outbound message has been checked for all known viruses by KPN IV-Scan, powered by MessageLabs. For further information visit: http://www.veiliginternet.nl _____________________________________________________________________________________________ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/