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We use Bayer Immuno 1 Troponin I method, and came across many falsely elevated results in the range 0.2 - 2.0 ug/L (analytical blunders). On repeat testing without re-centrifugation we get a result of < 0.1. We now double check all results in that range. Mohammad --- Peter Day <[log in to unmask]> wrote: > We at Prince of Wales Hospital in Sydney are using > the Dade RXL for troponin > I. We have been contacted by our users who > complained of some elevated > results. We then set to investigate and eventually > recentrifuged all > samples. We found no false negatives but 0.8% of our > high results were > falsely elevated (and sometimes very high). We use > lithium heparin and we > assume that the problem is most likely due to > micro-fibrin formation in > improperly mixed samples. We have added sonicated > fibrin to negative > troponin samples and did get elevated results. We > have also noticed a number > of samples had relatively high platelet counts in > the centrifuged plasma so > we cannot rule out platelet clumping although adding > platelet rich plasma to > troponin negative plasma only raised the troponin > slightly. > I would be interested to hear if other users have > noticed falsely elevated > troponins. > > -----Original Message----- > From: Jeff Slater > [mailto:[log in to unmask]] > Sent: Tuesday, 19 March 2002 5:08 > To: [log in to unmask] > Subject: Troponins on the Immulite 2000 > > > Does anyone have experience of the Troponin-I method > on the Immulite > 2000 ?. > I have had problems with establishing a reference > range and our > cardiologists would not be happy in using the assay > for stratification > purposes. They have also reported several > false-positives and false- > negatives, always after the specimens have been > discarded of course. > Any comments would be appreciated. > -- > Jeff Slater > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and > clinical > community working in clinical biochemistry. > Please note, archived messages are public and can be > viewed > via the internet. Views expressed are those of the > individual and > they are responsible for all message content. > > ACB Web Site > http://www.acb.org.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ > *********************************************************************************** > This email and the files transmitted with it are > confidential and intended solely for the use of the > individual or entity to whom they are addressed. If > you are not the intended recipient, you are not > permitted to distribute or use this message or any > of its attachments in any way. We also request that > you advise the sender of the incorrect addressing. > > This note also confirms that this email message has > been virus scanned and although no computer viruses > were detected, South East Health accepts no > liability for any consequential damage resulting > from email containing any computer viruses. > > *********************************************************************************** > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and > clinical > community working in clinical biochemistry. > Please note, archived messages are public and can be > viewed > via the internet. Views expressed are those of the > individual and > they are responsible for all message content. > > ACB Web Site > http://www.acb.org.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ ===== Dr. M A Al-Jubouri Consultant Chemical Pathologist __________________________________________________ Do You Yahoo!? Everything you'll ever need on one web page from News and Sport to Email and Music Charts http://uk.my.yahoo.com ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/