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A quicker way is to use "Lipoclear" from Statspin, this removes lipids in a few minutes. see http://www2.thecia.net/users/statspin/rp.html Tim [log in to unmask] -----Original Message----- From: p=NHS NATIONAL INT;a=NHS;c=GB;dda:RFC-822=ACB-CLIN-CHEM-GEN(a)JISCMAIL.AC.UK; Sent: 16 October 2002 20:35 To: p=NHS NATIONAL INT;a=NHS;c=GB;dda:RFC-822=ACB-CLIN-CHEM-GEN(a)JISCMAIL.AC.UK; Subject: Re: Hyperamylasaemia in DKA Another point to consider is the effect of lipaemia on the serum amylase result! In some cases you can simply let the sample stand in the fridge overnight and the chylomicrons will float to the top and then reanalyse the clear lower layer for amylase. cheers Trevor >>> Mohammad Al-Jubouri <[log in to unmask]> 16/10/02 12:23 am >>> Dear All A 17-year-old lady was admitted to our HDU with DKA last week end. She had abdominal pain and her serum amylase was elevated at 1483 IU/L (16 - 108). This is too high for DKA associated hyperamylasaemia and I would certainly consider acute pancreatitis either causing the DKA or coincidental with it. The admission sample was certainly grossly lipaemic which raises the possibility of hypertriglycerdiamia induced acute pancreatitis. My question is, what are amylase levels that can be attributed to DKA only and what is the cause of this elevation in the absence of acute pancreatitis? thanks Mohammad ===== Dr. M A Al-Jubouri Consultant Chemical Pathologist __________________________________________________ Do You Yahoo!? Everything you'll ever need on one web page from News and Sport to Email and Music Charts http://uk.my.yahoo.com ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ********************************************************************** ** This email and attachments have been scanned for content and viruses and is believed to be clean ** This email or attachments may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Canterbury District Health Board ********************************************************************** ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager at mailto:[log in to unmask] This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com ********************************************************************** ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/