Dear SPMers, I am able to do the above, but only at the price of deleting a mat file. Please let me know if I might be losing some information in doing this. I am doing two things: 1. flipping certain con images (one for each subject) so that they are reflected in the y-axis -> this apparently produces a mat file (with same name as reoriented con image). I do the flipping by choosing "display" and then "reorient images" after inserting -1 for x direction 2. I then create a mean image with each one of these flipped con images and another image (for each subject) -> this gives mean.img and mean.hdr. However, I get an error message if I try to do this averaging with the above-mentioned mat file in a subject's directory. So I delete the mat file and then I can create a mean image without any trouble. The error message is: "error using spm_mean_ui. images don't all have the same orientation and voxel size ..." However, without the mat file, spm_mean_ui is evidently happy to the do the averaging, so perhaps this is just a bug. Nevertheless, is deleting the mat file an acceptable solution or am I losing some information? Many Thanks Adam Aron - Brain Mapping Unit, Research Dept. Psychiatry, Addenbrookes Hospital, Cambridge, UK.