Dear SPM community: I have a methodology question about segmenting gray matter, white matter and CSF on MRI images. I have used SPM99 in Medx to create grey, white, CSF matter probability maps. Then I arbitrarily chose a "threshold" of 60% probability and binarized those voxels with 60% probability of being grey matter, white matter or CSF. Is this a valid method, compared to something like FSL? Does anyone know of any publications that validate this method? Regards Rob McClure