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| > I have 160 FMRI volumes of varying contrast that I would like to | > realign. Is it possible to use the Mutual Information cost function in | > the Realignment routine ? The way the defaults are set out seems to | > imply that MI is only available for Coregistration. | Coregistration is the same as realignment, so the answer is yes. The | warning "only for coregistration" is that you cannot use MI to normalise | images. Mutual information registration is not available by pressing the <Realign> button though. There may also be a few other practical difficulties - apart from speed. I haven't done any evaluations, but the current implementation in SPM99 may not have the sub-voxel accuracy that is necessary for time series realignment. I may need to do a bit more work on refining the interpolation. Best regards, -John %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%