Apologies to everyone, In my ignorance I previously sent a message as an attachment! Hope its now correct I was seeking your opinion on discrepant plasma versus serum calcium results that we have noted using Becton Dickinson 5ml vacutainers type SST & PST. This came to light from blood collected from subjects being investigated for ? hyperparathyroidism when both SST & PST blood samples were obtained at same venepuncture (we normally use PST for routine chemistries). Examples: Calcium mmol/L (Olympus method) PST 2.61 (albumin 28g/L) SST 2.39 (albumin 25) PST 2.29 18 SST 1.92 18 PST 2.80 17 SST 2.04 18 PST 2.74 21 SST 2.12 21 PST 2.30 18 SST 1.93 18 All specimens except the last PST were assayed within 1h of sample separation -- the last PST result was obtained following freezer storage. Has anyone noted similar with these types of "gel tubes" Thanks for your help Bob Ellis Rotherham DGH 01709 304103 Robert Ellis Principal Biochemist RDGH STOP %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%