Dear Dr van Bree
I also have an interest in this issue and wondered whether you had received
any useful feedback on this issue.
Yours sincerely
Ian Radford
Peter MacCallum Cancer Institute
Melbourne, Australia
> -----Original Message-----
> From: C.van Bree [SMTP:[log in to unmask]]
> Sent: Friday, 11 June 1999 18:39
> To: [log in to unmask]
> Subject: nuclear p53-immunoblot detection?
>
> Dear colleagues,
>
> I would like to detect nuclear and cytoplasmic levels of p53 in
> human tumour cells by immunoblot-analysis. I have tried the method
> described by Graeber et al. (Moll. Cell. Biol., 14: 6264-6277,
> 1994), but it is not working in my hands. Does anybody know an
> alternative method?
>
> Thanks,
> Chris van Bree, Ph.D.
> Department of Radiation Oncology
> Academic Medical Center, University of Amsterdam
> P.O. Box 22700, 1100 DE Amsterdam, The Netherlands
>
> Tel: +31 20 5668769
> Fax: +31 20 6091278
> E-mail: [log in to unmask]
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