Dear colleagues,
I would like to detect nuclear and cytoplasmic levels of p53 in
human tumour cells by immunoblot-analysis. I have tried the method
described by Graeber et al. (Moll. Cell. Biol., 14: 6264-6277,
1994), but it is not working in my hands. Does anybody know an
alternative method?
Thanks,
Chris van Bree, Ph.D.
Department of Radiation Oncology
Academic Medical Center, University of Amsterdam
P.O. Box 22700, 1100 DE Amsterdam, The Netherlands
Tel: +31 20 5668769
Fax: +31 20 6091278
E-mail: [log in to unmask]
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