Fellow Radiobiologists:
I am teaching A.S. degree students the basics in radiobiology. Is
there a source that discusses radiation (especially diagnostic)
interaction at the molecular level in terms of percentage of photons
(I understand that indirect is much more prevalent than direct),
expected damage and repair rates, involvement of histones vs
DNA, etc.?
The term "nuclear housekeeping": does that apply to damage
repair or does that apply to all activities in the nucleus?
Thank you for your response.
Date sent: Mon, 10 May 1999 11:34:33 +0000
Subject: Re: nuclear house keeping
From: "Joe Dynlacht" <[log in to unmask]>
To: [log in to unmask]
Send reply to: [log in to unmask]
> Tim,
>
> The expression of several of the nuclear matrix proteins identified to date
> may indeed vary with tissue-type. I don't pretend to be familiar with all
> of them, but with many, their content may also vary with differentiation
> status and proliferation status. This is particularly true of the nuclear
> lamins. Thus, using nuclear matrix proteins as housekeeping controls on
> westerns would probably not be a good idea. I can supply some references
> for you if you wish to investigate this further.
>
> Best Regards,
>
> Joe
>
> Joseph Dynlacht, Ph. D.
> Indiana University School of Medicine
> Department of Radiation Oncology
> Indiana Cancer Pavilion, RT 041
> 535 Barnhill Drive
> Indianapolis, IN 46202
> phone: (317) 278-3882
> FAX: (317) 278-0405
> e-mail: [log in to unmask]
> ----------
> >From: "Timothy J. Jorgensen" <[log in to unmask]>
> >To: [log in to unmask]
> >Subject: nuclear house keeping
> >Date: Fri, May 7, 1999, 5:19 PM
> >
> >Follow radiobiologists:
> >
> >I have a need to compare a protein's expression among different tissues on
> >a per cell basis. Since expression of most housekeeping genes varies
> >between tissues this has been problematic.
> >
> >It seems to me that nuclear matrix proteins should be constant from cell to
> >cell regardless of tissue (assuming all nuclei are roughly the same size).
> >Does anybody have any experience using nuclear matrix proteins as
> >housekeeping controls on westerns? Or does anyone have an alternative
> >suggestion for solving my problem?
> >
> >Thanks for your help.
> >
> >Tim Jorgensen
> >
> >Timothy J. Jorgensen, Ph.D.; Associate Professor
> >Department of Radiation Medicine
> >Georgetown University Medical Center
> >Lombardi Cancer Center/TRB room E212A
> >3970 Reservoir Road, N.W.
> >Washington, D.C. 20007-2197
> >
> >telephone 202-687-1810
> >facsimile 202-687-2221
> >e-mail [log in to unmask]
> >
> >Lombardi Cancer Center web site: http://lombardi.georgetown.edu/index.html
>
Vincent Fried
Medical Imaging Instructor
Lima Technical College
4240 Campus Dr
Lima, OH 45804
(419).999.8809
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The other man's grass may be greener, but it still needs mowing. James Dobson
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