Hi Miranda
We use fine grade silica gel, cut-up the sponge finely and cover it
completely with silica gel (small tubes), then use a chelating resin to
extract the DNA from the dessicated tissue. No need to freeze the sponge,
although we usually keep the silica gel samples frozen in the lab for extra
safety.
After a year or so storage the DNA is only slightly degraded (i.e. not
perfect), but appears to be better than the buffer method of Michelle K-B.
Christine Diaz and Belinda Alvarez both used the silica gel method. Perhaps
email them directly. Gert Woerheide is using the technique in our lab, so he
could probably also give you some advice if needed.
cheers, John Hooper
-------------------------------------------------------
Dr J.N.A. Hooper
Acting Director, Natural Environment Program
Queensland Museum
P.O. Box 3300
SOUTH BRISBANE, QLD, 4101, AUSTRALIA
fax +61-7-3846-1226 or 3846-1918
phone +61-7-3840-7722 or 0412-695-592
email [log in to unmask] <mailto:[log in to unmask]>
http://www.qmuseum.qld.gov.au <http://www.qmuseum.qld.gov.au>
-------------------------------------------------------
-----Original Message-----
From: Miranda Sanders [SMTP:[log in to unmask]]
Sent: Tuesday, November 09, 1999 9:16 AM
To: [log in to unmask]
Subject: DNA extraction
Hi everyone,
I will be collecting some sponges for DNA extraction on our next
expedition
to Papua New Guinea at the end of this month. I want to preserve the
specimens for a couple of weeks until my return to the lab where I
can
begin extractions. Any suggestions on optimal preservation methods
for this
purpose ? (Freezing is probably not a viable option.)
Any recommendations would be much appreciated.
Thanks,
Miranda
Miranda Sanders
Dept. of Chemistry & Biochemistry
University of California, Santa Cruz
1156 High St.
Santa Cruz, CA 95064, USA
Tel: (831) 459-5641
Fax: (831) 459-2935
email: [log in to unmask]
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
|