Dear Ioanna,
just to add my two cents, you'll want to create two thresholded nifti files with SPM's "Results" tab (Display: Save -> Thresholded SPM): one for the contrast [group 1 > group 2], and one for [group 2 > group 1]. Then, you can add both nii-files as overlays in your visualization software of choice. However, some visualization tools only allow for adding 1 overlay at a time (e.g. the BrainNet Viewer: https://www.nitrc.org/projects/bnv/). In these cases, I like to create a two-sided nifti file by simply subtracting the two images from each other, i.e. here [group 1 > group 2] - [group 2 > group 1]. You can do this fairly easily using SPM's "ImCalc" - I'll attach an SPM batch that does this to this email. You can load this batch via the "Batch" tab.
I hope this helps!
All the best,
Philipp
--
Dr. Philipp Kuhnke
Postdoctoral Researcher
University of Leipzig, Germany
%-----------------------------------------------------------------------
% Job saved on 01-Oct-2023 01:15:18 by cfg_util (rev $Rev: 7345 $)
% spm SPM - SPM12 (7771)
% cfg_basicio BasicIO - Unknown
%-----------------------------------------------------------------------
matlabbatch{1}.spm.util.imcalc.input = {
'\data\spmT_0001.nii,1'
'\data\spmT_0002.nii,1'
};
matlabbatch{1}.spm.util.imcalc.output = 'Group1_vs_Group2_twoSided.nii';
matlabbatch{1}.spm.util.imcalc.outdir = {'\data\derivatives'};
matlabbatch{1}.spm.util.imcalc.expression = 'i1-i2';
matlabbatch{1}.spm.util.imcalc.var = struct('name', {}, 'value', {});
matlabbatch{1}.spm.util.imcalc.options.dmtx = 0;
matlabbatch{1}.spm.util.imcalc.options.mask = -1;
matlabbatch{1}.spm.util.imcalc.options.interp = 0;
matlabbatch{1}.spm.util.imcalc.options.dtype = 64;
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