Hi,
Thanks.
I realized that you imported particles from CryoSPARC.
Because I don't know how metadata conversion to/from other software
works, I cannot investigate this further.
Can you re-extract particles from run_data.star and make sure
the coordinates make sense?
In general, "barcode stripes" appear when particles go outside a
micrograph. This might be genuine (i.e. re-extracting a particle
close to an edge into a larger box) but can also be caused by very
bad motion trajectories.
Best regards,
Takanori Nakane
On 2023/04/08 1:16, Matt Martin wrote:
> Hi Takanori,
>
> I just sent those files over to you. Also, if it helps, we used Relion's implementation of motioncorr for the micrographs input into polishing, using CPUs instead of GPUs. We used a dose per frame of 1.5 and an EER fractionation of 40.
>
> Matt Martin
>
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