Hi Dieter,
My guess (just a guess) is that this will depend on the size of the protein. The larger, the less likely you will see a large improvement. The smaller (particularly if < 150 kDa), the more likely.
Best regards,
Basil
> Am 06.01.2023 um 09:37 schrieb Dieter Blaas <[log in to unmask]>:
>
> Hi,
> we obtained a 4 A map of a protein by using just the first 30 frames from 60 frame-movies. Can we expect a significant improvement by including all frames?
>
> if so, is it the right way to re-extract the particles from the above refinement (with 30 frames) from MotionCorr / CftFind runs, where the 60 frames were included, and re-refine?
>
> thanks for hints, best, Dieter
> apologies if this mail was sent twice!
> ------------------------------------------------------------------------
> Dieter Blaas,
> Max Perutz Laboratories
> Medical University of Vienna,
> Inst. Med. Biochem., Vienna Biocenter (VBC),
> Dr. Bohr Gasse 9/3,
> A-1030 Vienna, Austria,
> Tel: 0043 1 4277 61630,
> Mobile: 0043 699 1942 1659
> e-mail: [log in to unmask]
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