Hi,
You should have saved power spectra during Motion Correction
("Save sum of power spectra?").
Best regards,
Takanori Nakane
________________________________________
差出人: Collaborative Computational Project in Electron cryo-Microscopy <[log in to unmask]> が Aninda Dutta <[log in to unmask]> の代理で送信
送信日時: 2022年7月19日 16:12
宛先: [log in to unmask]
件名: [ccpem] RELION ctffind error
While working with the SPA tutorial dataset, after motion correction, we are finding the following error while doing CTF estimation using ctffind 4.1-
in: /home/user/relion/src/ctffind_runner.cpp, line 156
ERROR:
ERROR: You are using --use_given_ps, but there is no rlnCtfPowerSpectrum label in the input micrograph STAR file.
=== Backtrace ===
/usr/local/bin/relion_run_ctffind(_ZN11RelionErrorC2ERKNSt7__cxx1112basic_stringIcSt11char_traitsIcESaIcEEES7_l+0x77) [0x5653a6b96fb7]
/usr/local/bin/relion_run_ctffind(_ZN13CtffindRunner10initialiseEv+0x1f23) [0x5653a6b88333]
/usr/local/bin/relion_run_ctffind(main+0x49) [0x5653a6b6f049]
/lib/x86_64-linux-gnu/libc.so.6(__libc_start_main+0xe7) [0x7fc6afe44c87]
/usr/local/bin/relion_run_ctffind(_start+0x2a) [0x5653a6b7221a]
==================
ERROR:
ERROR: You are using --use_given_ps, but there is no rlnCtfPowerSpectrum label in the input micrograph STAR file.
It would be a real help if anyone could help us with a way-out . Thank you in advance !
Aninda Dutta
Junior Research Fellow
ICMR- National Institute of Cholera and Enteric Diseases
Kolkata, India
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