Dear relion users,
I am just starting to learn how to use relion for subtomogram averaging. I reconstruct and pick particles using etomo/imod.
I followed this tutorial to prepare data for subtomogram averaging in relion (https://www3.mrc-lmb.cam.ac.uk/relion//index.php/Sub-tomogram_averaging)
I generated files for 2 tomograms as described in the tutorial, run the relion_prepare_subtomograms.py then tomo_reconstruct_ctfs.sh (using relion2)
I am stuck in 'Sub-tomogram extraction' step. I am confused which version of relion the tutorial is referring. I can't find where to load .coords file for both tomograms.
I also tried to follow the tutorial for subtomogram averaging in relion 4 page but it is not explained how to prepare input data ( coords_tomo.star ? with coordinates) (https://relion.readthedocs.io/en/latest/STA_tutorial/index.html)
Thank you for your help,
Cheers,
Fadwa
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