Hi,
Didn't the program say the following?
Read an EER gain file GainReference.gain.
Taking inverse and re-scaling (when necessary).
Written XXX/gain-reference.mrc. Please use this file as a gain reference when processing the converted movies.
Best regards,
Takanori Nakane
On 2022/02/14 22:09, Yehuda Halfon wrote:
> Hi all,
>
> I'm trying to use relion_convert_to_tiff command, and I have some questions:
> I use the following command:
> relion_convert_to_tiff --i movies.lst --o /path/out/ --gain GainReference.gain --eer_grouping 16 --eer_upsampling 1
> I get the tif in the end, but when I try to do motion correction on them with the gain file I get "Invalid Spider file: GainReference.gain". In the
> documentation it says: "Due to the different meanings of the gain reference for EER and TIFF, you have to take the inverse of the EER gain reference
> before processing the resulting TIFF files. The tool performs this conversion when the EER gain reference is specified in the --gain option."
> Am I missing something? Do I need to convert the gain also?
>
> Yehuda
>
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