Hi Sjors and RELION team,
Thank you so much for making a lot of improvements to RELION-4.0, I really enjoyed the speed of new VDAM algorithm and the integration of Topaz particle picking. But I had a error when I was testing relion_it.py (error message below), I thought it could be due to the python environment on my computer, just wondering what's the best practice to solve this kind of issue.
Thanks,
Bing-Rui
----------------------
$ relion_it.py
RELION_IT: -------------------------------------------------------------------------------------------------------------------
RELION_IT: script for automated, on-the-fly single-particle analysis in RELION (>= 4.0)
RELION_IT: authors: Sjors H.W. Scheres, Takanori Nakane & Colin M. Palmer
RELION_IT:
RELION_IT: usage: ./relion_it.py [extra_options.py [extra_options2.py ....] ] [--nogui]
RELION_IT:
RELION_IT: -------------------------------------------------------------------------------------------------------------------
RELION_IT:
RELION_IT: launching GUI...
Traceback (most recent call last):
File "/usr/local/RELION/relion-4/bin/relion_it.py", line 1025, in <module>
main()
File "/usr/local/RELION/relion-4/bin/relion_it.py", line 1019, in main
tk_root = tk.Tk()
NameError: global name 'tk' is not defined
------------------------
On 9/23/21, 11:04 AM, "Sjors Scheres - MRC LMB" <[log in to unmask]> wrote:
Dear EM-ers,
We are pleased to announce that RELION-4.0 is now available for public
beta testing. A big thank you to all those who helped testing it.
RELION-4.0 comes with:
1) a completely new approach to subtomogram averaging
2) a new algorithm, called Variable-metric Gradient Descent with Adaptive
Moments (VDAM) for relion refine, which among other benefits results in
much faster and better 2D classifications
3) Automated 2D class selection
4) An improved framework, called Schemes, for automated execution of
workflows, e.g. for on-the-fly processing.
5) Enhanced integration with the new pipeliner approach that is being
developed for the CCPEM software suite
(https://ccpem-pipeliner.readthedocs.io/en/latest/)
RELION-4.0 remains proudly open-source, and is completely free for all
users. Download the code from: https://github.com/3dem/relion/tree/ver4.0
Read its new documentation at: https://relion.readthedocs.io/en/release-4.0
Best regards,
The RELION team (https://relion.readthedocs.io/en/release-4.0/Team.html)
PS: Two publications on RELION-4.0 are pending: one on its new subtomogram
averaging approaches; another one on the improvements for single-particle
analysis. We will post them on bioRxiv once they are ready.
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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