Hi,
Probably your contrast settings for relion_display are wrong.
Set 0 to Min, Max and Sigma contrast.
Best regards,
Takanori Nakane
On 2021/08/10 7:59, Irin Tom wrote:
> Hi all
>
> I am new to cryoEM processing and was trying to process a data set using RELION 3.1.2. I noticed that even my best 2D classes look a bit different.
> They look shiny which I do not understand why. Also I doubt why the surrounding of my particles (in the mask) is looking weird too.
> I would really appreciate any suggestions or advise.
> Thank you
> Irin
>
>
> image.png
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