On Sun, 2021-08-01 at 13:36 +0100, Axel Innis wrote:
> Dear all,
>
> We have two cryo-EM maps at ~3.0 Å resolution:
>
> Map A: a ribosomal complex with bound Ligand 1
> Map B: a ribosomal complex with bound Ligand 1 and Ligand 2
>
> The addition of Ligand 2 induces conformational changes within the complex containing Ligand 1, but Map B is a mixture
> of two states: the state observed in Map A and a second state, whose structure we would like to determine, which we
> will call C.
>
> As a result:
>
> C = (B – Ax) / y
>
> Where x and y are the fraction of states A and C in map B, respectively.
>
>
Difference maps can be calculated in Coot using Calculate -> Map Tools... -> Make a Difference Map...
Paul
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