Hi Lora,
you will have to perform global alignment searches as your particles
will need to be aligned on the imposed symmetry axis.
Best,
Pranav
--
Pranav Shah
Postdoctoral Research Fellow.
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive, Oxford OX3 7BN,
UK
On Fri, Jun 4, 2021 at 8:28 PM Lora Sinha <[log in to unmask]> wrote:
>
> I want to do a symmetry average of my reconstruction. Is there a way to align the images on the new symmetry aligned reference by modifying the euler angles, instead of rerunning global refinement on the new reference, followed by symmetrized refinement?
>
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