Hi Carlos,
Map modification implemented in Phenix could be helpful too. You could also check if the map resolution is overestimated.
Hope this helps.
Best regards,
Min Woo Sung
> On May 20, 2021, at 8:10 AM, Carlos Fernandes <[log in to unmask]> wrote:
>
> Dear all,
>
> I am facing some problems with sharpening in post-processing.
> My current map is about 3.2 Å resolution, which I obtained after CTF-Refinement and Polishing.
> When I determine that factor B is estimated automatically, post-processing tells me a factor B of -81 is applied, but very little sharpening is done. Attached, I put an image with a focus on the helices of my structure to better visualization (I applied high dust Chimera function in the maps).
> I did some tests applying progressive B-factor values (-120; -140; -165; -190), but I was unable to visualize a typical helices sharpening.
> Has anyone had problems like that? Any tips?
> Thanks in advance,
> Carlos.
>
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