Dear CCPEM colleagues,
has anyone experience with holey formvar only grids? My experiment works
only on a formvar coating, not on carbon. Formvar with an additional
carbon coating also does not work for me.
So far I have tried continously coated grids with formvar, but I have
troubles getting thin ice on these grids during blotting/plungin with
the Vitrobot. I verify my experiments in advance using fluorescence and
a confocal microscope, from which point on my grids are constantly in
solution.
So far I couldnt find grids that have a holey formvar only film. Does
anyone has ever used / purchased / manufactured such grids?
Thanks to all in advance
Best
Dario
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