Hi,
> I wish to redetermine the CTF using the isotropic apix
This is not necessary.
Please read: https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Pixel_size_issues#What_should_I_do_if_the_pixel_size_turned_out_to_be_wrong.3F
Best regards,
Takanori Nakane
On 2021/02/01 22:22, Schmid, Michael F. wrote:
> Hi-
>
> I have processed some data with (nominally) 0.52 Å/pixel, up to the end of the Post-processing step. I get a 2.6Å map. Using well-determined x-ray
> coordinates with accurate unit cell parameters, I find that the (significantly) best “Average map value” in Chimera Fit-to-Map is obtained when the
> map has an apix of 0.5114. I wish to redetermine the CTF using the isotropic apix that I found (equivalent to mag, of course), but the only option in
> “CTF refinement” in Relion is “anisotropic magnification”. I don’t see a place at this step to just enter the apix value I want. I hope I don’t have
> to go all the way back to “CTF estimation”. Is there a .star file location for this, and will changing it have unintended consequences somewhere else?
>
> Thanks,
>
> Mike
>
>
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