Hi Elliot,
This is a bug. It's usually pretty harmless, which seems to be your case
too as the map looks good. The last iteration value is incorrect for one
of the halfmaps.
HTH,
Sjors
> Dear CCPEM community,
>
> I have a question regarding symmetry search for helical reconstruction.
>
> When running an auto-refine job with symmetry search, it seems the two
> half-maps converge to identical parameters (rise/twist) up until the
> second-to-last iteration. However, they diverge in the very last iteration
> (see screenshots below). To note: the resolution is great, the estimated
> accuracies look good, and – most importantly – the volume looks great.
>
> The question is: which is it? Are the averaged parameters those to be
> reported? Or rather does this indicate potential residual heterogeneity in
> the dataset?
> Any input would be appreciated !
>
>
> Warm regards,
> Elliot J. Crooks
>
> At 2nd the last iteration:
>
>
> At last iteration:
>
> _______________________________________________________________________________________
>
>
> Elliot J. Crooks, M.S.
> Ph.D. Candidate – Amyloid & cryo-EM laboratory
> Stony Brook University (NY)
> [log in to unmask]
> +1 (631)459-7998
> elliotcrooks.com <http://elliotcrooks.com/>
>
>
>
>
>
>
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--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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