Hi,
The regrouping job can only merge existing groups to form a larger group.
It cannot split a big group into smaller ones.
Best regards,
Takanori Nakane
On 2020/12/16 15:46, Franken, Linda wrote:
> Dear Takanori,
>
> I tried to regroup the particles by doing first a classification (without alignment) to have a model.star file to work from followed by selection and
> regroup particles. But then when I select a number of classes or all classes it always groups the particles in 10 groups, independent of the number of
> groups that I like to have. I tried with relion 3.0 and 3.1 and this does not make a difference. Any idea how I could create smaller groups?
>
>
> Best wishes,
>
> Linda
>
>
>
> ------------------------------------------------------------------------------------------------------------------------------------------------------
> *Van:* Takanori Nakane <[log in to unmask]>
> *Verzonden:* dinsdag 15 december 2020 11:46
> *Aan:* Franken, Linda; [log in to unmask]
> *Onderwerp:* Re: [ccpem] error Zero sum of weights
> Hi,
>
>> I see, that makes sense. Do you think starting the run over in relion 3.1 would solve the issue?
>
> If the crash is due to a bug in RELION 3.0 that has been fixed in 3.1, yes.
> But there are many other causes so the problem can persist.
>
> Anyway, we no longer support 3.0.
>
>>> _rlnGroupScaleCorrection #4
>>> 1 group_003 2851 0.864271
>>> 2 group_001 6884 0.775772
>>> 3 group_005 2839 1.095779
> Why is your group so large?
>
> If a group contains particles with very different noise level,
> the program will crash.
>
> Best regards,
>
> Takanori Nakane
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