Hi,
> Hi, I have a question about relion_estimate_gain, as I could not find a full description of the procedure anywhere. For K3 camera with about 1 e/A2/frame images, how many total frames are needed to get an estimation as good as experimental one?
It depends on e/px, not e/A2. It also depends on how "uniform" your movies are.
If in doubt, just average all frames you have.
> Or will it always be a bit worse than experimental gain file?
Again, this depends on how many electrons you put in the experimental gain file.
I myself use an experimental gain by default. Only when I see artifacts, I try gain estimation.
> Also, how do relion_estimate_gain results compare to xmipp_movie_estimate_gain (pubmed.ncbi.nlm.nih.gov/29551714/) results, as the algorithms are probably different?
I don't know. RELION's implementation simply averages given frames and take the inverse of it.
I am sure there are better ways, but in practice, it does not seem to make a big difference.
I have once had a bug where the gain reference was applied in the opposite way round (divide or multiply).
The resolution remained the same before and after the fix.
Best regards,
Takanori Nakane
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