Dear David and all,
Speaking from as SAMBA user
How have existing users of the Samba/DNA nudge gone about verifying this method?
The samba can use VTM/UTM diluted 1/2 with their bespoke buffer.
We ran 20neg and 20pos left over patient samples.
Then we double swabbed 20 patients with verbal consent (I would have preferred written but we were mid-surge so teams felt it was an acceptable ask for the time).
We ran IQCs - there are a few available.
We are currently running some Qnostics standards through all of our networks methods to compare LOD.
Do your high throughput lab reference methods use dry or transport medium swabs?
At time our lab used VTM.
No they use dry, but into their own bespoke buffer.
Have you asked for double swabbing on patients ? - see above. Only a VERY limited number and only once all other avenues had shown good performance.
how are dry flocked swab types transported to the testing areas without a transport tube?
So there are dry swabs with tubing that can be used to transport. (Dry swab Purflock by medical wire is an example).
But we actually have a dry swab and the swabber puts it into the SAMBA bespoke falcon tube. Which we transport using a 250ml Pot to keep it upright - which we recycle to reduce plastic throwaway use.
This is the big benefit of our POCT location - staff come to us to get the pot/tube/swab - and bring the test back to us.
We allocate out their 24hour pots - so we know we wont over-run the device capability. This has also helped us manage stock pressures.
Hope this helps
Katy
Katy Heaney
POCT Specialty Lead, Consultant Biochemist
Berkshire & Surrey Pathology Services
Qpoint EQA Scheme Director
Frimley Health NHS Foundation Trust
Tel: 07557 865 201
Email: [log in to unmask]
Notice of A/L; 24th – 28th August .
-----Original Message-----
From: ACB Point-of-Care Testing <[log in to unmask]> On Behalf Of David Seeruthun
Sent: 14 August 2020 08:56
To: [log in to unmask]
Subject: Re: Samba II DNA nudge
Many thanks for all of your replies. Very useful to see how POCT colleagues are adapting to the challenges!
I looked at both technologies and while they are marketed as POCT devices not in traditional lab settings, there are numerous pre analytical steps involved which I feel are too complicated for our nursing staff to perform.
I note that both methods utilise dry flocked swabs that need to be instead into a lysis buffer reagent prior to testing rather than using traditional transport medium swabs. Here at SASH we already have a Cepheid GeneXpert for Influenza and SARS CoV-2 using transport medium swabs. There are challenges with reagent allocation hence looking at alternatives to keep up with the winter demands! The good news is Cepheid are producing a tetravalent cartridge that encompasses A/B/RSV/SARS CoV-2 but I still feel that it wont be enough!
How have existing users of the Samaba/DNA nudge gone about verifying this method? Do your high throughput lab reference methods use dry or transport medium swabs? Have you asked for double swabbing on patients ? and how are dry flocked swab types transported to the testing areas without a transport tube?
Many thanks again all
Kind regards
Dave
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