Sorry, way off topic:
Does anyone have an estimate for the flow rate one would typically use for the cold nitrogen stream passing over a protein crystal in a standard data collection?
Background: Our nitrogen “generator” has gone belly-up and the vendor no longer services it, so I’m testing the feasibility of using the boil-off from a liquid nitrogen tank to provide the gas to support a short data collection (this nitrogen gas would serve as the feedstock into our helium cryostat). But I don’t know the flow rate required, so I can’t calculate if one tank has enough nitrogen to support a day or so of data collection. There are flow meters for the warm and cold stream on the nitrogen generator, but these flow meters have no apparent units anywhere on them, so I have no idea of the rate at which the gas would be consumed.
Thanks for any useful tidbits.
And for those of you in the US, best wishes for a happy “Holy crap, even MORE fireworks?!?!!” Day
Pat
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Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102 USA
(215) 762-7706
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