Correction: It should read "with the detector almost but not quite
hitting the source."
On 02.07.20 17:13, Navdeep Sidhu wrote:
> Alexander Blake wrote a nice chapter on small-molecule crystallization
> in this book, if you run into problems in the crystallization stage:
>
> Alexander Blake. Crystal growth and evaluation (Chapter 3). In Clegg,
> William (Ed.) Crystal Structure Analysis: Principles and Practice. 2nd
> Edition. IUCr/Oxford, 2009
> <https://www.oxfordscholarship.com/view/10.1093/acprof:oso/9780199219469.001.0001/acprof-9780199219469>;
> <https://global.oup.com/academic/product/crystal-structure-analysis-9780199219476>.
>
> With the detector almost but not quite hitting the detector, one can
> typically collect all the data one needs on a home Cu source. Overloads
> can often be a problem---as has been pointed out out for
> synchrotrons---even on a home source. But they can usually be dealt with
> easily by modifying exposure, collecting lower- and higher-angle data
> separately, leaving some overlap between the two regions (see "Intensity
> level", Blake, Ch. 6.7.1, p. 88 in book above) and scaling. In extreme
> cases, one may need to lower the generator power.
>
> The nice thing about the slow pace of data collection on a home source
> is that one can think while doing the collection, thus catching and
> correcting most problems before it's too late. In Ch. 7.6 in the book,
> Blake also discusses some other problem cases. (The whole book's a great
> read.)
>
> Cheers,
> Navdeep
>
>
>
>>>> ---
>>>> On 01.06.20 23:50, Jiyuan Ke wrote:
>>>> > Hi Everyone,
>>>> >
>>>> > I want to crystallize a small organic molecule. I have
>>>> very limited
>>>> > experience in small molecule crystallography. I found
>>>> that the Crystal
>>>> > Screen HT from the Hampton research is good for both
>>>> small molecule and
>>>> > macromolecule crystallization. Plan to set up a sitting
>>>> drop screen just
>>>> > like setting up protein crystallization. I don’t know
>>>> if this is the
>>>> > proper way to do it. Is the MRC sitting drop 2-well
>>>> plate (HR3-083) used
>>>> > for protein crystallization good for small molecule
>>>> crystallization? Are
>>>> > there any special plates used for small molecule
>>>> crystallization? Is
>>>> > room temperature ok or not?
>>>> >
>>>> > For data collection, can I use the beamline for protein
>>>> crystals to
>>>> > collect data on small molecule crystals? Larger
>>>> oscillation angle,
>>>> > shorter exposure, reduced beam intensity?
>>>> >
>>>> > For structure determination, is SHELXL the preferred
>>>> software for
>>>> > solving small molecule structures?
>>>> >
>>>> > If anyone has experience in small molecule
>>>> crystallography, please
>>>> > help. Thanks!
>>>> >
>>>> > Best Regards,
>>>> >
>>>> > --
>>>> >
>>>> > *Jiyuan Ke, Ph.D.*
>>>> >
>>>> > *
>>>> > *
>>>> >
>>>> > Research Investigator
>>>> >
>>>> > H3 Biomedicine Inc.
>>>> >
>>>> > 300 Technology Square, Floor 5
>>>> >
>>>> > Cambridge, MA 02139
>>>> >
>>>> > Phone: 617-252-3923
>>>> >
>>>> > Email: [log in to unmask]
>>>> <mailto:[log in to unmask]>
>>>> <mailto:[log in to unmask]
>>>> <mailto:[log in to unmask]>>
>>>> >
>>>> > Website: www.h3biomedicine.com
>>>> <http://www.h3biomedicine.com/>
>>>> <http://www.h3biomedicine.com/>
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