Dear CCP4 users,
I'm working on the another nucleic acid duplex structure. After several
stages of rebuilding and refinement I observed something like a "shadow"
of the second duplex position. Have you any ideas to explain this
phenomena? Should I place the second molecule with occupancy completed
to the first one? Or maybe is it a kind of disorder in crystal lattice?
Data were collected to 1.02A using synchrotron radiation (processed with
XDS), but I have another datasets collected to lower resolution (from
Zn-SAD) with visible similar effects. Refinement was done using Refmac
(R/Rfree 21.5/24.0). Data not appeared to be twinned, but Xtriage
detected pseudo-translational symmetry.
Thank you for any advices.
Rafal
|----------------------------------------------|
|Rafal Dolot, Ph.D. |
| |
|Polish Academy of Sciences |
|Centre of Molecular and Macromolecular Studies|
|Department of Bioorganic Chemistry |
|Macromolecular Crystallography Team |
|Sienkiewicza 112 |
|90-363 Lodz, Poland |
|Phone: +48(42)6803215 |
|Cell: +48 502897781 |
|----------------------------------------------|
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
|