Hi,
> What you mean by the 'top directory' is the directory where you type "relion" to launch and where output folders get created, correct? If so, yes, the movies are in the 'top directory.'
Yes.
This is a current problem is Warp. Of course this itself does not cause your
crash but the folder becomes messy. Also having thousands of files in
a folder slows down some file systems.
Best regards,
Takanori Nakane
> I will check on the WARP export again - sure the lack of gain-ref is definitely problematic.
>
> Thanks again.
>
> H
>
>
>
>
> ________________________________________
> From: Takanori Nakane [[log in to unmask]]
> Sent: Wednesday, June 24, 2020 6:03 PM
> To: Furukawa, Hiro; [log in to unmask]
> Subject: Re: [ccpem] unable to load micrograph #... in Relion
>
> Hi,
>
> > _rlnMicrographMovieName FoilHole_21124063_Data_20044996_20044998_20200322_092713_fractions.tiff
>
> This means the movies must be in the top directory of the project.
> Are you really doing this?
>
> The image size suggests K3 but the metadata does not contain the gain reference.
> Is this also intended?
>
> Best regards,
>
> Takanori Nakane
>
> On 2020/06/24 22:42, Hiro Furukawa wrote:
>> Thanks for your reply.
>>
>> The failed ones are always the same sets of movies and I confirmed that they are present.
>> I placed the tiff movies, "motion" directory created by WARP containing metadata for each movies and all of the tiff movies in the Relion directory:
>>
>> data_general
>>
>> _rlnImageSizeX 4092
>> _rlnImageSizeY 5760
>> _rlnImageSizeZ 30
>> _rlnMicrographMovieName FoilHole_21124063_Data_20044996_20044998_20200322_092713_fractions.tiff
>> _rlnMicrographBinning 1.00000
>> _rlnMicrographOriginalPixelSize 0.85600
>> _rlnMicrographDoseRate 2.04000
>> _rlnMicrographPreExposure 0
>> _rlnVoltage 300
>> _rlnMicrographStartFrame 1
>> _rlnMotionModelVersion 0
>>
>>
>> Hiro
>>
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