Hi,
Perhaps the center of the particles and the center of the reference
were far off? In such cases, even if you make the "initial offset range"
larger, RELION cannot find the right center. This is because the
initial standard deviation of the translation prior is 10 angstroms.
You can change this to X angstroms by "--offset X" to Refine3D.
I will add this point to the wiki.
Best regards,
Takanori Nakane
> Hi Takanori,
> Thank you again for your reply, I did try everything suggested on relion
> wiki page, and I also tried with a global circular mask, those attempts
> all have failed.
> But, interestingly, the data set I was dealing with is a subunit on a big
> complex, so I changed the reextract coordinates a little bit, and the mask
> accordingly, this strategy bypassed the error, really don't know why, but
> it works.
> Thank you,
> Yours sincerely,
> Linhua Tai
>
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