Happy New Year CCPEM!

I have tried to refine beam-tilt in combined data set Mics1-3 (it is 
actually the same scope, just different realignments due to technical 
issues). At the beginning I imported three movies sets, set them 
-opticsGroup1-3 and the joined them.

  + Adding new optics_group with name: opticsGroup2
  + Renumbering group opticsGroup2 from 1 to 2
  + Adding new optics_group with name: opticsGroup3
  + Renumbering group opticsGroup3 from 1 to 3
  Written: JoinStar/job013/join_movies.star
  Done!

The joined star file point to 3 optic groups, but all the movies are 
actually pointed to opticsgroup 1. None to 2 or 3.

job013% more join_movies.star

# version 30001

data_optics

loop_
_rlnOpticsGroupName #1
_rlnOpticsGroup #2
_rlnMtfFileName #3
_rlnMicrographOriginalPixelSize #4
_rlnVoltage #5
_rlnSphericalAberration #6
_rlnAmplitudeContrast #7
opticsGroup1            1 K3_mtf.star     0.870000   300.000000     
2.700000     0.100000
opticsGroup2            2 K3_mtf.star     0.870000   300.000000     
2.700000     0.100000
opticsGroup3            3 K3_mtf.star     0.870000   300.000000     
2.700000     0.100000

data_movies

loop_
_rlnMicrographMovieName #1
_rlnOpticsGroup #2
Mics1/FoilHole_25846986_Data_25831184_25831186_20191125_1647_fractions.mrc 
            1
Mics1/FoilHole_25846986_Data_25846178_25846180_20191125_1647_fractions.mrc 
            1
Mics1/FoilHole_25846987_Data_25831184_25831186_20191125_1648_fractions.mrc 
            1
.
.
.
.
Mics3/FoilHole_28969832_Data_28973514_28973516_20191202_0142_fractions.mrc 
            1
Mics3/FoilHole_28969833_Data_28973470_28973472_20191202_0142_fractions.mrc 
            1
Mics3/FoilHole_28969833_Data_28973514_28973516_20191202_0143_fractions.mrc 
            1



Which then results that all the movies are treated as the same.
(base) 
[koubatom@max-display001]/beegfs/cssb/user/koubatom/LASV_C_R3p1/CtfRefine/job268% 
ls
asymmetric_aberrations_optics-group_1.eps                     job.star   
       particles_ctf_refine.star
beamtilt_delta-phase_iter-fit_optics-group_1_N-3.mrc          
logfile.pdf      RELION_JOB_EXIT_SUCCESS
beamtilt_delta-phase_lin-fit_optics-group_1_N-3.mrc           
logfile.pdf.lst  run.err
beamtilt_delta-phase_lin-fit_optics-group_1_N-3_residual.mrc  Mics1      
       run.hostfile
beamtilt_delta-phase_per-pixel_optics-group_1.mrc             Mics2      
       run.out
default_pipeline.star                                         Mics3      
       run_submit.script
job_pipeline.star                                             note.txt


It seems the error is at the JoinMovie step.

Is there anything I am missing in order to avoid this next time?

Thanks for your advice

Tomas

PS: I guess I can somehow change the opticsgroup name according to the 
Mics1-3 name for the CtfRefinement job imput star files.

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