Hi,
What is the resolution "before per particle CTFRefine"?
Can you cite the FSC table in run_model.star?
Best regards,
Takanori Nakane
> Hi,
>
> I am struggling with a situation of tilt data refinement. The refinement
> before per particle CTFRefine runs fine and ends with converged map. Both
> half maps look fine from the final round but the merged map
> run_class001.mrc has nothing in it, it's all dust. Any ideas why?
>
> Auto-refine: Refinement has converged, stopping now...
> Auto-refine: + Final reconstruction from all particles is saved as:
> Refine3D/job014/run_class001.mrc
> Auto-refine: + Final model parameters are stored in:
> Refine3D/job014/run_model.star
> Auto-refine: + Final data parameters are stored in:
> Refine3D/job014/run_data.star
> Auto-refine: + Final resolution (without masking) is: 8.91892
> Auto-refine: + But you may want to run relion_postprocess to mask the
> unfil.mrc maps and calculate a higher resolution FSC
> --
>
> ______________________
>
> Regards,
>
> Humayun Sharif, PhD
>
> Research Fellow | Wu Lab
> BCMP, Harvard Medical School
>
> Immune Disease Institute
> PCMM, Boston Children's Hospital
> 3 Blackfan Circle | Boston
> MA, 02115, USA
> P:+1-857-218-9051
>
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