Dear colleagues,

We are developing a new validation method that takes EM maps and models 
into account. In order to understand the potential, the applicability and 
the limitations of the method we are looking for good test cases, which 
turn out to be surprisingly hard to find.

What we are looking for are EM structures with serious modelling errors, 
e.g. register errors (model sequence out-of-register with the map), 
connectivity errors (between (non-)adjacent secondary structure elements), 
directionality errors (e.g., a helix built backwards), substantial 
stretches of mistraced residues, etc.

The structures should be publicly available in PDB (model) and EMDB (map), 
they should be at 4.0Å resolution or better, and ideally there should be a 
higher resolution improved structure (EM or X-ray).

If the modelling errors have been confirmed and discussed in the 
literature that is ideal, but we are also interested if this is not the 
case.

Feel free to reply either in confidence to me (mailto:[log in to unmask]), 
or to the entire list.

Many thanks in advance for any examples you can think of!

PS: apologies for cross-posting to two other mailing lists.

--Gerard

---
Gerard J. Kleywegt, EMBL-EBI, Hinxton, UK
Head of Molecular and  Cellular Structure
[log in to unmask] pdbe.org emdb-empiar.org
PA: Roisin Dunlop    [log in to unmask]

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