Hi,
Joining polished (shiny) particles are easy.
1. Go to Polish folder within the dataset2 project.
2. Make a symbolic link to dataset1's Polish/jobXXX by FULL PATH.
(A link by relative path sometimes causes unexpected problems
in RELION when it tries to expand paths)
3. Make sure the optics group name in shiny.star from the dataset 1
is different from the dataset 2.
4. Join shiny.star from dataset 1 and dataset 2.
(You don't have to import; just go up .Node directory to see
the file)
5. Refine & PostProcess
6. Run CtfRefine with "anisotropic magnification: Yes" to make sure
the magnification is really the same.
Best regards,
Takanori Nakane
On 2019/12/05 22:37, Humayun Sharif wrote:
> Hello,
>
> Could anyone please comment on what would you do in the following scenario.
>
> We have both datasets taken on same microscope and with same settings.
>
> Dataset1: Motioncor and CTF done on *Relion 3.0 *and Refinement and
> Polish on *3.1*
> Dataset2: All jobs done on *3.1 *
>
> We would like to import Dataset1 (refined particles) in Dataset2 Relion
> session and join the star files for further processing and reconstruction.
>
>
>
> Thanks
>
> --
>
> ______________________
>
> Regards,
>
> Humayun Sharif, PhD
>
> Research Fellow | Wu Lab
> BCMP, Harvard Medical School
>
> Immune Disease Institute
> PCMM, Boston Children's Hospital
> 3 Blackfan Circle | Boston
> MA, 02115, USA
> P:+1-857-218-9051
>
>
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