Hi,
First of all, is "Relion 3.0 beta" typo of
RELION 3.1 beta? Otherwise, you should use at least RELION 3.0.8.
> the high frequency signal is abnormal (why?), but the curve still looks
Probably you used half maps from intermediate iterations in PostProcess.
Please use the converged half maps and repeat subsequent steps.
Best regards,
Takanori Nakane
On 2019/12/03 20:25, Minglei Zhao wrote:
> Dear Microscopists,
>
> I encountered a weird behavior of FSC for an apoferritin dataset
> processed using Relion 3.0 beta:
>
> After 3D refinement and post process, everything looks good:
>
>
> After CtfRefine (first beam tilt, then anisotropic magnification, then
> defocus), refined again, and post process, the high frequency signal is
> abnormal (why?), but the curve still looks OK before 0.7(1/A). Estimated
> resolution improved a little bit:
>
>
> After Bayesian Polishing, refined again using mask and solvent-flattened
> FSC, the estimated resolution is 1.9 A, FSC from Refine3D job looks fine:
>
>
> However, using the same mask in the previous refinement to filter and
> sharpen the map, the FSC curve is messed up:
>
>
> Any obvious things I did wrong?
>
> Thanks for your attention.
>
> Minglei
>
>
> ------------------------------------------------------------------------
>
> To unsubscribe from the CCPEM list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>
########################################################################
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
|