Hi ,

Just wonder if anyone can help please:

We at Bradford Royal Infirmary RadioPharmacy have been using a downsized version of ITLC-SG for Myoview QC.

Since the supply problem of ITLC-SG paper, GE has changed the method to using ITLC-SA.

Our QA wants us to stick to product spc methods, ie ITLC-SA. BUT I am struggling to obtain consistent results following the GE spc .

So the questions here are:

Is the GE spc ITLC-SA method workable?

Dose the ITLC-SA( Agilent) stripes need heat activating?. My understanding is you do NOT need to heat ITLC-SA, But the label on the box does states heating is required? I have contacted Agilent and still waiting for an answer.

How long does it take to get the tank to equilibrate. We are using 20cm stripes and the tank has a proportional bigger space in total. I may be wrong here, but I found it may take up to more than two hours to stand any chance of getting any decent results. ( I have heard about pre-saturation of tank using mobile phase soaked materials , but this will entail use of much more organic solvents?)


PS: I have co-run our mini ITLC-SG AND the Sep-pak c18 systems alongside the ITLC-SA, the first two tend to be fairly consistent. The ITLC-SA system gives hopelessly  unpredictable results.

Your help and comments will be much appreciated.


Jim

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