Hi,
Going back many years when the Varian paper was available we never encountered any issues with Myoview test, when Varian stopped and we had to use the ITLC-SA from agilets I noticed that the was a difference with the paper, once the solvent had run and you took out the paper it dries very quickly and it is extremely fragile making cunt and count a challenge.
We were getting issues with result so we tried to resolve any issues that would cause rcp fail result. We don't dry our paper but we store it in such a way that it does not absorb moisture in the air as this causes test to fail, the moment we start work which is before 05.30am we immediately mix our solvents such that by 6.am we start testing giving ample time for equilibration the amount of time it requires to reach solvent front is 30-40mins.
When I have not had enough staff I have used the biodex myoview strips.
Hope this helps.
-----Original Message-----
From: Radiopharmacy UK [mailto:[log in to unmask]] On Behalf Of Jim Lo
Sent: 21 November 2019 11:04
To: [log in to unmask]
Subject: ITLC for Myoview
Hi ,
Just wonder if anyone can help please:
We at Bradford Royal Infirmary RadioPharmacy have been using a downsized version of ITLC-SG for Myoview QC.
Since the supply problem of ITLC-SG paper, GE has changed the method to using ITLC-SA.
Our QA wants us to stick to product spc methods, ie ITLC-SA. BUT I am struggling to obtain consistent results following the GE spc .
So the questions here are:
Is the GE spc ITLC-SA method workable?
Dose the ITLC-SA( Agilent) stripes need heat activating?. My understanding is you do NOT need to heat ITLC-SA, But the label on the box does states heating is required? I have contacted Agilent and still waiting for an answer.
How long does it take to get the tank to equilibrate. We are using 20cm stripes and the tank has a proportional bigger space in total. I may be wrong here, but I found it may take up to more than two hours to stand any chance of getting any decent results. ( I have heard about pre-saturation of tank using mobile phase soaked materials , but this will entail use of much more organic solvents?)
PS: I have co-run our mini ITLC-SG AND the Sep-pak c18 systems alongside the ITLC-SA, the first two tend to be fairly consistent. The ITLC-SA system gives hopelessly unpredictable results.
Your help and comments will be much appreciated.
Jim
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