Thanks Basil and Takanori!
I guess this just won't work then. As an aside, I didn't expect a 3D to
be any better than 2D; I was just trying to avoid the need for manual
picking and 2D classification on a new sample. (LoG picking hasn't
worked well for me on hollow structures like apoferritin).
Thanks again,
-jh-
On 10/2/19 1:13 PM, Takanori Nakane wrote:
> Hi,
>
> The 3D reference must have the same grey level as the micrographs.
> A map from atomic models will not work probably.
>
> This feature is useful when you have a map from the same dataset
> (e.g. a preliminary map from a subset of micrographs) and/or very
> similar datasets (same grid, different session).
>
> In my experience, a 3D reference is not necessarily better than
> 2D references. Remember that the reference is strongly low pass
> filtered. Many different views look similar at low resolution
> unless the protein is very elongated.
>
> Best regards,
>
> Takanori Nakane
>
> On 2019/10/02 19:42, John Heumann wrote:
>> Sorry, I should clarify that I'm trying to autopick using Relion 3.07.
>>
>> Regards,
>> -jh-
>>
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--
John M. Heumann
Department of Molecular, Cellular, and Developmental Biology
347 UCB, University of Colorado
Boulder, CO 80309-0347
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