Hi,
The 3D reference must have the same grey level as the micrographs.
A map from atomic models will not work probably.
This feature is useful when you have a map from the same dataset
(e.g. a preliminary map from a subset of micrographs) and/or very
similar datasets (same grid, different session).
In my experience, a 3D reference is not necessarily better than
2D references. Remember that the reference is strongly low pass
filtered. Many different views look similar at low resolution
unless the protein is very elongated.
Best regards,
Takanori Nakane
On 2019/10/02 19:42, John Heumann wrote:
> Sorry, I should clarify that I'm trying to autopick using Relion 3.07.
>
> Regards,
> -jh-
>
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