Hi,
Just out of curiosity, how large was the beam tilt and
what is the resolution after CtfRefine?
Best regards,
Takanori Nakane
On 2019/10/21 18:37, Minglei Zhao wrote:
> Dear Microscopists,
>
> It turned out that the problem is a misalignment of the microscope which introduced a significant beam tilt. After Relion CTF refine with beam tilt, the dip disappeared.
>
> Thanks again for your input.
>
> Best,
> Minglei
>
>> On Oct 17, 2019, at 3:47 PM, Minglei Zhao <[log in to unmask]> wrote:
>>
>> Dear All,
>>
>> Thank you so much for your quick and helpful responses. Will update later when the problem is resolved.
>>
>> Best regards,
>> Minglei
>>
>>> On Oct 17, 2019, at 3:38 PM, Takanori Nakane <[log in to unmask]> wrote:
>>>
>>> See also:
>>> https://www3.mrc-lmb.cam.ac.uk/relion//index.php?title=Pixel_size_issues
>>>
>>> Takanori
>>>
>>> On 2019/10/17 20:47, Takanori Nakane wrote:
>>>> Hi,
>>>> This is most likely caused by a huge beam tilt or a wrong pixel size.
>>>> Less likely causes are higher-order aberrations and anisotropic
>>>> magnification.
>>>> Run CtfRefine in RELION 3.1 with 3rd and 4th order aberration
>>>> options enabled and see phase residual plots in logfile.pdf.
>>>> Best regards,
>>>> Takanori Nakane
>>>> On 2019/10/17 20:44, Minglei Zhao wrote:
>>>>> Dear Microscopists,
>>>>>
>>>>> We were testing a newly installed Krios with K3 and energy filter and came across this weird FSC curve after Relion Postprocessing.
>>>>>
>>>>> The sample is apoferritin and the slit of energy filter was left out during data collection.
>>>>>
>>>>> Any idea what might cause the problem?
>>>>>
>>>>> Thank you for your attention.
>>>>>
>>>>> Best regards,
>>>>> Minglei
>>>>>
>>>>> red: phase randomized curve
>>>>> green: unmasked
>>>>> blue: masked
>>>>>
>>>>>
>>>>> ------------------------------------------------------------------------
>>>>>
>>>>> To unsubscribe from the CCPEM list, click the following link:
>>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>>>>
>>>> ########################################################################
>>>> To unsubscribe from the CCPEM list, click the following link:
>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>>
>>> ########################################################################
>>>
>>> To unsubscribe from the CCPEM list, click the following link:
>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>
>
> ########################################################################
>
> To unsubscribe from the CCPEM list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>
########################################################################
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
|