See also:
https://www3.mrc-lmb.cam.ac.uk/relion//index.php?title=Pixel_size_issues
Takanori
On 2019/10/17 20:47, Takanori Nakane wrote:
> Hi,
>
> This is most likely caused by a huge beam tilt or a wrong pixel size.
> Less likely causes are higher-order aberrations and anisotropic
> magnification.
>
> Run CtfRefine in RELION 3.1 with 3rd and 4th order aberration
> options enabled and see phase residual plots in logfile.pdf.
>
> Best regards,
>
> Takanori Nakane
>
> On 2019/10/17 20:44, Minglei Zhao wrote:
>> Dear Microscopists,
>>
>> We were testing a newly installed Krios with K3 and energy filter and
>> came across this weird FSC curve after Relion Postprocessing.
>>
>> The sample is apoferritin and the slit of energy filter was left out
>> during data collection.
>>
>> Any idea what might cause the problem?
>>
>> Thank you for your attention.
>>
>> Best regards,
>> Minglei
>>
>> red: phase randomized curve
>> green: unmasked
>> blue: masked
>>
>>
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