Hi,
This is expected. RELION 3.1 does not copy same particles many times
(as long as they are ordered side-by-side in the input STAR file).
Best regards,
Takanori Nakane
> I’m running a 3D classification of a set of ~75k particles generated using
> relion_particle_symmetry_expand on of a set of ~25k particles with C3
symmet
> ry. This is something I’ve done successfully several times in previous Reli
> on versions.
>
> However, when I run this in Relion 3.1, Class3D reports copying ~25k
> particles from optics group 1 to the scratch disk rather then the ~75k
> that are actually in the input particle file (see below)
>
> Copying particles to scratch directory:
> /local_fast/scratch/relion_volatile/
> 5/ 5 sec
> ............................................................~~(,_,">
> For optics_group 1, there are 25123 particles on the scratch disk.
>
> Should I be worrying about this, or is it just recording the three
> symmetry expansions of each particle as a single particle for downstream
> optical refinement purposes ?
>
> Laurence
>
> ----------------------------------------------------
> Laurence H. Pearl PhD FRS FMedSci
>
> Professor of Structural Biology
> Genome Damage and Stability Centre
> University of Sussex, Brighton, BN1 9RQ, UK
>
> Phone +44-(0)1273 678 349
> ----------------------------------------------------
> "Live Modestly and do Serious Things .. "
> - Dorothy Crowfoot Hodgkin
> ----------------------------------------------------
>
>
>
>
>
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